Measurement
14QE

Part:BBa_I744102

Designed by: Thomas Segall-Shapiro   Group: iGEM07_Rice   (2007-07-18)

Tetracycline sensor with medium-strength promoter

Tc -> EYFP

This biobrick is intended to report on the levels of Tetracycline inside a cell. It uses part J23114 (relative strength 256) to drive synthesis of tetR, which determines the sensitivity of the circuit. EYFP is produced for measurement. A transfer function is available in the experience section.


For more information go to: [http://2007.igem.org/Rice/Project_A:_Phage_Project#Experimental_System_and_Circuit_Design Rice iGEM 2007 Constructs]

Tet sensor.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None